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Sharon G. Greenberg, Peter Davies, A Preparation of Alzheimer Paired Helical Filaments that Displays Distinct Ï„ Proteins by Polyacrylamide Gel Electrophoresis, Proceedings of the National Academy of ...
Proteins were extracted from wheat meal or flour in 0.1 M acetic acid, 3 M urea and 0.01 M CTAB and fractionated in columns of cross-linked Sepharose in the same solvent. An heterogeneous fraction of ...
To begin, the proteins under study are prepared for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and consequently loaded onto the gel for separation based on their ...