The discovery of restriction enzymes nearly forty years ago revolutionized the study of DNA and accelerated our ability to both sequence and synthesize genomes. These enzymes opened the path to a ...

These tools to help design restriction enzyme experiments are frequently visited on the NEB website, www.neb.com; making them available as free downloads brings their accessibility closer to the ...

cDNA Library Construction sans Restriction Enzymes Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every ...

Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix.

Enzymes Involved in Molecular Cloning There are three types of enzymes used for cloning. These include restriction endonucleases, polymerases, nucleases, ligases, kinases, and phosphatases.

Restriction sites are particular sequences of genetic letters recognised by proteins called restriction enzymes that, in nature, cut up viral genetic material in bacterial cells.